We are sorry for the issues that you are having with a bacterial stab that you received from us. Please verify that you have followed the Recipient Instructions and used the correct antibiotic and growth temperature, which are found under Growth in Bacteria on the plasmid webpage. If you continue to experience difficulties with your stab, please email help@addgene.org with the completed information below and a scientist from our Technical Support Team would be happy to provide further assistance.
Order number:
Plasmid ID number:
Antibiotic and concentration:
Growth Temperature:
Were you able to recover colonies when streaked from the stab onto a LB plate (yes/no):
Did a single colony grow in liquid culture (yes/no):