We apologize if you have experienced an issue using a plasmid that you received from Addgene.
As a non-profit plasmid repository, we are unable to functionally test the plasmids we distribute and cannot troubleshoot problems with transfection, protein expression, or other functional issues. We recommend consulting the publication associated with the plasmid for additional information.
I have obtained an unexpected restriction digest or sequencing result with the plasmid I have received
We will be happy to address any restriction digest or sequencing concerns that you might have. Please send an email to help@addgene.org and include the following information:
- Addgene order number
- Addgene plasmid ID number
- Data demonstrating the issue
For a diagnostic restriction digest of the plasmid, please include
- DNA ladder with band sizes labeled
- Digested sample labeled with the enzyme(s) used
- Undigested sample
For a sequencing result, please include
- The .ab1 file (also called a trace file or chromatogram)
- The sequence of the primer used to obtain the result
The DNA concentration or volume is not as expected
Addgene ships Cloning Grade DNA as 2 µg of DNA at a concentration of 100 ng/μl ±5 ng/μl (as measured by Nanodrop) provided on the tube you receive. If there is an issue with the DNA concentration or volume, please send an email to help@addgene.org and include the following information:
- Addgene order number
- Addgene plasmid ID number
- DNA concentration and the method used to measure this
I am unable to recover bacterial colonies after transformation of my DNA prep
Please see our Bacterial Transformation protocol for a detailed protocol and troubleshooting tips. The appropriate E. coli strain and antibiotic to use for selection can be found under the “Growth in Bacteria” section on each plasmid page.
For further assistance, please send an email to help@addgene.org and include the following information:
- Addgene order number
- Addgene plasmid ID number
- DNA concentration and volume used for transformation
- Antibiotic and concentration used for selection
- Incubation temperature and time