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What type of Quality Control does Addgene perform?

For most plasmids, Addgene will perform single pass sequencing to validate the identity of the insert, any mutations indicated during the deposit process (ex: mismatches, truncations, insertions, etc), and the frame of tags or fusion proteins. These sequencing results will sometimes, but not always, cover the full insert.

Addgene will contact you if there are any discrepancies between your cloning data and your sequencing results prior to making your plasmid available online. When the analysis is complete, the sequencing results are posted online for the community.

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