Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target or to confirm expected labeling across a tissue panel in one of the antibody’s recommended applications. The specific experiments performed varies for each antibody catalog item. Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived equivalent to ensure potency and specificity have been retained.
Potential experiments performed include:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)
For more details on each of these assays, please see our Antibody Production page. To learn which specific QC experiments were performed on your lot, please contact help@addgene.org.
Please see the Institute for Protein Innovation (IPI) Help Center article for specific information on these antibodies.