Within two weeks of receiving your new Addgene plasmid you should create a glycerol stock. You can also transform and purify plasmid DNA for storage at -20 °C. To create your glycerol stocks, please follow Addgene’s protocol below.
-
Streak Bacteria for Single Colonies – use your bacterial stab to streak bacteria on a plate and isolate single colonies.
-
Inoculate an Overnight Liquid Culture – using a liquid culture will allow you to grow enough bacteria for plasmid DNA purification and for creating glycerol stocks.
-
Create a Glycerol Stock and Store at -80 °C – most plasmids are stable for years when stored as bacterial glycerol stocks.