Addgene antibodies are supplied as a liquid on blue ice to avoid unnecessary freeze-thaw cycles. If you intend to use the antibody immediately, you may store it at 4˚C or on ice upon receipt. For long-term storage, consult the product information on our website for the optimal storage temperature, as each antibody may have different recommended storage temperatures.
If you are freezing your antibody, first centrifuge the sample for 30 seconds at 10,000 x g to draw down the liquid and then prepare single-use aliquots. Store single-use aliquots at -20˚C in a manual defrost freezer. Avoid multiple cycles of freeze-thaw.
Addgene antibodies are provided in PBS containing 1mM sodium azide. This buffer is not compatible for use in live cells and will interfere with conjugation. For applications such as these, the buffer can be removed by dialysis or gel filtration.
Within 60 days you should verify that your antibody performs as expected. We will be happy to help address any issues you encounter with your antibody. Please email email@example.com with your:
- Order number
- Antibody ID
- Antibody Application of interest
We will respond as soon as possible to assist you.