Addgene ships Cloning Grade DNA as 2 µg of DNA in 10 mM Tris-Cl, pH 8.5. This DNA preparation has been validated through next-generation sequencing and is ready for use in downstream cloning applications. The DNA will be provided to you at 100 ng/μl ±5 ng/μl (as measured by Nanodrop) and the concentration will be listed on the tube you receive. We recommend briefly spinning down the tube prior to use.
Cloning Grade DNA should not be used for direct transfection into cells.
DNA can be stored in the tube you receive at 4°C for short-term storage, or -20°C for long-term storage. You may also want to consider creating a glycerol stock, which could be used for additional plasmid DNA isolation, if necessary.
While Cloning Grade DNA preparations have undergone sequencing validation, you may wish to further verify your stock by diagnostic restriction digest or Sanger sequencing.
Please see our Cloning Grade DNA Troubleshooting article for assistance with troubleshooting any issues with the DNA you receive.