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Which plasmids were used for AAV packaging?

AAV preparations are generated in HEK293 cells either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities. Transfections are performed using the transfer plasmid, a plasmid encoding rep and serotype-specific cap, and a plasmid encoding adenoviral helper sequences.

More information regarding our general AAV production protocol and quality control can be found on our Viral Production page.

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