Which plasmids were used for lentiviral packaging? Lentivirus is produced with the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. For complete details on how our viruses are produced through viral service, visit our Viral Production page. Related articles Which plasmids were used for AAV packaging? What is the composition of the virus formulation (i.e., what solution is my virus in)? Addgene quality control protocols I could not find a plasmid on Addgene’s website. What do I do now? How do I process my Addgene plasmid (bacterial stab)?