Which plasmids were used for lentiviral packaging? Lentivirus is produced with the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. For complete details on how our viruses are produced through viral service, visit our Viral Production page. Related articles Addgene quality control protocols Experiment protocols How do I process my Addgene plasmid (bacterial stab)? Which plasmids were used for AAV packaging? Does Addgene generate custom virus preparations?