Which plasmids were used for lentiviral packaging? Lentivirus is produced with the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. For complete details on how our viruses are produced through viral service, visit our Viral Production page. Related articles Addgene quality control protocols What is the composition of the virus formulation (i.e., what solution is my virus in)? Experiment protocols Who is authorized to sign the MTA? How do I ship plasmids to Addgene?