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Kits shipped on dry ice

Store the glycerol stocks in the kit at -80°C upon receipt. Take precautions to minimize freeze-thaw cycles. Keep the plate on dry ice to prevent thawing. To access a plasmid, use a sterile pipette tip to puncture the seal above an individual well and then streak a portion of the glycerol stock onto an agar plate to isolate single colonies. To patch the hole, use sterile tape or a portion of a fresh aluminum seal.

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