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Long-term plasmid storage

Within 2 weeks of receiving your new Addgene plasmid you should create a glycerol stock, or prep purified plasmid for storage at -20°C. To create your glycerol stocks, please follow Addgene’s protocol below.

  1. Streak Bacteria for Single Colonies - use your bacterial stab to streak bacteria on a plate and isolate single colonies.

  2. Inoculate an Overnight Liquid Culture - using a liquid culture will allow you to grow enough bacteria for plasmid DNA purification and for creating glycerol stocks.

  3. Create a Glycerol Stock and Store at -80°C - most plasmids are stable for years when stored as bacterial glycerol stocks.

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