Within 2 weeks of receiving your new Addgene plasmid you should create a glycerol stock, or prep purified plasmid for storage at -20°C. To create your glycerol stocks, please follow Addgene’s protocol below.
Streak Bacteria for Single Colonies - use your bacterial stab to streak bacteria on a plate and isolate single colonies.
Inoculate an Overnight Liquid Culture - using a liquid culture will allow you to grow enough bacteria for plasmid DNA purification and for creating glycerol stocks.
Create a Glycerol Stock and Store at -80°C - most plasmids are stable for years when stored as bacterial glycerol stocks.