Addgene Help Center

Return to

Plasmid purification from kit

After proper storage of your new Addgene kit you may wish to purify the plasmid DNA out of the bacteria. Addgene has provided an example of a manual miniprep protocol for isolating plasmid DNA from an overnight liquid culture.

  1. Inoculate an Overnight Liquid Culture - using a liquid culture will allow you to grow enough bacteria for plasmid DNA purification.

  2. Isolate Your Plasmid DNA - many companies sell miniprep or maxiprep kits for easy plasmid isolation. Addgene also provides a protocol for plasmid purification without a kit.

Powered by Zendesk