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Plasmid purification from kit

After proper storage of your new Addgene kit you may wish to purify the plasmid DNA out of the bacteria. Addgene has provided an example of a manual miniprep protocol for isolating plasmid DNA from an overnight liquid culture.

  1. Inoculate an Overnight Liquid Culture - using a liquid culture will allow you to grow enough bacteria for plasmid DNA purification.

  2. Isolate Your Plasmid DNA - many companies sell miniprep or maxiprep kits for easy plasmid isolation. Addgene also provides a protocol for plasmid purification without a kit.

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